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NF-κB and AP-1 activity in MARC-145, MARC-2a, and MARC-N cells. Cells were transfected with the secreted alkaline phosphatase (SEAP) reporter vectors: pNFκB-SEAP and pAP1-SEAP. The culture supernatants were collected and analyzed for SEAP activity and represented as relative fluorescence unit (RFU) per mL. The data represent the results of three independent experiments in triplicates, and error bars indicate standard deviations of the means. P < 0.05 (*); P < 0.01 (**).
