Skip to main content
. 2014 Jan 13;111(4):E435–E444. doi: 10.1073/pnas.1311121111

Fig. 4.

Fig. 4.

AICAR and metformin do not suppress glioma proliferation by inducing chronic energy crisis. (A) HPLC analysis shows the energy content of T98G glioma cells treated with AMPK agonists. (B) O2 consumption (OCR) and (C) glycolysis (ECAR) of T98G glioma cells treated with AICAR or metformin. OCR (D) and ECAR (E) were also measured in control (nt) and AMPKβ1 shRNA T98G cells. nt, nontarget. (F) Proliferation of control and Rho-0 T98G cells treated with AICAR or metformin. Inset shows RT-PCR analysis of mitochondrial transcripts [cytochrome oxidase II (CoxII) and NADH dehydrogenase 4 (ND4)] in control and Rho-0 cells. Data shown is representative of at least three independent experiments. *P ≤ 0.001. Error bars in A–F represent mean ± SD.