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. 2014 Jan 13;111(4):1427–1432. doi: 10.1073/pnas.1317838111

Fig. 2.

Fig. 2.

Teleost germ cells express the Lhcgrba. (A–E) Zebrafish testis. (F–I) Gilthead seabream testis. (A and F) Hematoxylin-eosin staining of testes. (B and G) Lhcgrba immunoblots of whole testis with (+) or without (−) preadsorbed antibody (C and H). In situ hybridization of lhcgrba expression in testes. (D, E, and I) Immunostaining of Lhcgrba (red) superimposed to the DAPI nuclear counterstain (blue), using the sole Lhcgrba antibody. Lc, Leydig cells; Sc, Sertoli cells; Spg, spermatogonia; SpcI, spermatocyte type 1; SpcII, spermatocyte type 2; Spd, spermatid; and Spz, spermatozoa. (Scale bars, A–C and F–H, 20 µm; D, E, and I, 10 µm.)