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. 2014 Jan 13;111(4):1427–1432. doi: 10.1073/pnas.1317838111

Fig. 5.

Fig. 5.

The Lhcgrba-activated cAMP/PKA signaling pathway drives spermatid differentiation into spermatozoa in vitro. (A) Effect of the Lhcgrba (Lhcgrba Ab) and Fshra (Fshra Ab) antibodies on rLh (10 ng/mL)-stimulated spermatozoa differentiation in Senegalese sole SLL cell extracts. The addition of FSK (10 ng/mL) rescues the Lhcgrba Ab inhibition. (B) Effect of different inhibitors on rLh-induced spermatozoon differentiation. In A and B, data are the increment in the percentage of spermatozoa with respect to time 0. (C) Relative expression of rsph1, sept7a, odf2l, and cull3 quantified by qRT-PCR in SLL extracts treated with rLh in the presence or absence of the MDL and H-89 inhibitors. In all panels, data are the mean ± SEM (n = 3–4). *P < 0.05; **P < 0.001, with respect to cells not treated with rLh or treated with rLh or inhibitor alone (brackets). SPZ, spermatozoa.