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. 2014 Jan 14;111(4):1586–1591. doi: 10.1073/pnas.1316283111

Fig. 3.

Fig. 3.

(A) Expression of srfA was measured spectro-fluorimeterically at three different time points in srfA-cfp–labeled strains: QS-proficient (QS+) B. subtilis PS216 and signal-deficient (QSS−) PS216ΔcomQ incubated with or without purified ComX. (B) The dehydrogenase activity of B. subtilis PS216 and signal-deficient PS216ΔcomQ grown with or without ComX (0.05 fraction) was determined after 4, 6, and 8 h of incubation. (C) Growth of QS+ B. subtilis PS216 and signal-deficient (QSS−) PS216ΔcomQ in competence medium without and with purified ComX (0.05 fraction) was monitored spectro-photometrically at 650 nm. (D) Growth of QSS− B. subtilis PS216ΔcomQ and double-mutant QSS−A− PS216ΔcomQΔsrfA deficient in ComX and surfactin production in the absence (control) and presence of ComX (0.05 fraction) was monitored spectro-photometrically at 650 nm. Data are presented as the mean of biological triplicates with SE indicated for every time point.