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. 2014 Jan 13;111(4):1479–1484. doi: 10.1073/pnas.1323318111

Fig. 1.

Fig. 1.

RICH mice support a robust P. falciparum infection. Humanized mice were injected hydrodynamically with plasmids encoding human IL-15 (50 µg) and Flt3L (10 µg) on day zero. Beginning 1 d after plasmid injection, mice were injected i.p. daily with 1 mL human RBCs. After 7 d of RBC supplementation, mice were infected i.v. with 5 × 106 ring-stage 3D7 parasites and given daily RBC supplementation throughout the experiment (n = 10). (A–C) Human RBC and immune cell reconstitution before parasite infection (7 d after plasmid injection). Shown are representative CD235ab vs. forward scatter (FSC) profiles of whole blood (A), human CD45 (hCD45) vs. mouse CD45.1 (mCD45) staining profiles of PBMCs (B), and human CD56 vs. CD14 and CD3 vs. CD19 staining profiles gating on human CD45+ cells (C). The numbers indicate the percentage of cells in the gated regions. The mean ± SEM (n = 10) are shown in Results. (D) Quantification of parasitemia in RICH mice at different time points. Data are shown as percentage (mean ± SEM) of iRBCs among total RBCs and are a compilation from two independent experiments with five mice in each experiment.