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. 2014 Jan 13;111(4):1604–1609. doi: 10.1073/pnas.1315567111

Fig. 2.

Fig. 2.

Onecut1/2 regulate production of Isl-1-positive neurons downstream of Coup-tfs. (A) OE of HA-tagged Onecut2 in p2 neurospheres resulted in prolonged production of Isl-1 + neurons (n = 3). The control with nuclear-localized EGFP is also shown. (Scale bar, 50 μm.) (B) Repression of Onecut1 and Onecut2 by specific artificial miRNAs reduced the production of Isl-1 + neurons and eliminated the Coup-tf-KD phenotype in p1 neurospheres (n = 3). GFP fluorescence stemmed from the lentiviral construct containing the indicated miRNA/shRNA. (Scale bar, 50 μm.) Results are shown as mean ± SEM. NS, P > 0.05; *P < 0.05.