Fig. 3.

Overexpression of LGP2 inhibits radiation-induced killing. D54 cells were stably transfected by full-size p3xFLAG–CMV10–LGP2 (LGP2) or control p3xFLAG–CMB10 (Flag). Selected clones were propagated, plated in six-well plates, and irradiated at 0, 5, and 7 Gy. (A) Crystal violet staining of survived colonies 12 d after irradiation of cells, transfected with Flag (Upper) or LGP2 (Lower). (B) Quantification of survival fraction of mock-transfected and LGP-transfected cells (Methods). Representative Western blot of stable Flag and LGP2 clone is inserted into B.