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Six KaiB monomers bind one phosphorylated KaiC hexamer. Native MS of KaiC and KaiCB complexes. (A) KaiC forms hexamers. (B) Upon mixing KaiB and KaiC under standard conditions of the in vitro oscillator, KaiC6B6 and KaiC6B1 complexes are formed. (C) Nearly complete formation of KaiC6B6 is achieved when an excess of KaiB is added and incubated at reduced temperature. (Insets) Transformed spectra, where m/z is converted to mass.
