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. 2014 Jan 13;111(4):1421–1426. doi: 10.1073/pnas.1318445111

Fig. 1.

Fig. 1.

Genetic screen yields two unique drosha alleles that are defective in miRNA biogenesis. (A) Schematic of Drosha protein indicates two RNase III domains (RIII) and double-stranded RNA binding domain (gray square), as well as locations of mutations. The drosha∆,E859K allele deletes 147 amino acids (∆) and contains a missense mutation at residue 859. (B–E) Eye discs from third-instar larvae containing WT (B), droshaR662X (C), droshaR1113X (D), and drosha∆,E859K (E) clones stained with anti-Drosha antibodies (magenta). Clones are indicated by the absence of GFP. (Scale bar, 10 μm.) (F) Northern blots of total RNA from third-instar larvae of indicated genotypes probed for miR-2b, let-7, miR-125, and U6 snRNA (loading control). (G) In vitro processing of pri-let-7 by WT, E859K, ∆, E859K+∆, or dominant negative (DN) Drosha proteins. Length of incubation is indicated in minutes.