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. 2004 Mar 18;23(7):1627–1635. doi: 10.1038/sj.emboj.7600155

Figure 3.

Figure 3

ARTS specifically binds XIAP in vitro. In vitro binding was tested using the GST pull-down assay. (A) COS-7 cells were transient transfected with pcDNA myc XIAP construct. GST pull-down was performed using GST–ARTS and Western blot was caried out with anti-XIAP and (B) with anti-myc. (C) COS-7 cells were transient transfected with AU5-ARTS, AU5-ARTSΔC or AU5-H5. GST pull-down was performed using GST–XIAP and Western blot was carried out with anti-AU5. Binding to glutathione beads alone served as a negative control. (D) Direct binding of ARTS to XIAP is shown in an in vitro assay using GST–XIAP and purified recombinant ARTS protein. GST protein served as a negative control.