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. 2004 Mar 18;23(7):1627–1635. doi: 10.1038/sj.emboj.7600155

Figure 6.

Figure 6

Upon apoptotic induction, ARTS binds higher levels of XIAP in a caspase-independent manner. (A) NRP-154 cells without apoptotic treatment, or treated with 100 μM etoposide for 3 and 6 h were used for co-immunoprecipitation using anti-ARTS antibodies. Western blot analysis was carried out with anti-XIAP antibodies. (B.I) COS-7 cells without apoptotic treatment, or treated with 1 μM STS for 1 and 3 h were used for co-immunoprecipitation using anti-ARTS antibodies. COS-7 cells were also treated with BOC (caspase inhibitor). (B.II) XIAP levels in the same lysates used for IP (B. I) are shown. During apoptotic induction, both NRP-154 cells and COS-7 cells show a significant increase of ARTS binding to XIAP. ARTS–XIAP interactions do not depend on caspase activity and do not occur as a consequence of apoptosis.