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. 2014 Jan 13;111(4):1443–1448. doi: 10.1073/pnas.1317165111

Fig. 2.

Fig. 2.

Differentiation of the ESR1 promoter sequence has led to changes in transcription efficiency. (A) Predicted loss or gain of transcription factor binding sites. SNPs are indicated by white circles, insertions by black circles, and deletions by gaps, compared with the ZAL2 haplotype. The sequence alteration that potentially confers binding of PBX1 and several other transcription factors is located within a region corresponding to the mammalian DNase hypersensitive region of the ESR1 promoter, indicating that these changes have likely occurred within an active region for transcription factor binding. (B) In HeLa cells, the ZAL2m construct inserted upstream of luciferase (luc) resulted in significantly more expression compared with the ZAL2 construct (P = 0.007). (C) E2 added to the cell culture (1 µM) did not alter transcription efficiency for either construct (E2 × construct interaction, P = 0.941).