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. 2014 Jan 13;111(4):E465–E473. doi: 10.1073/pnas.1320319111

Fig. 3.

Fig. 3.

Increased thymocyte apoptosis leads to increased intrathymic TGFβ. (A–D) C57BL/6 mice were injected i.p. with 2 or 20 mg/kg DEX or DMSO control, and thymi were examined 18 h later. (A) FACS plots show AnnexinV versus 7AAD staining. (B) Intrathymic amounts of total TGFβ1 in whole thymi determined by ELISA. (C) mRNA expression of tgfb1, tgfb2, tgfb3, Smad7, and tgif1 in whole thymi; results are presented relative to DMSO control. (D) DC, macrophages (Macs), and TECs were FACS-sorted from thymi of mice treated with DMSO (white bars) or 20 mg/kg DEX (black bars) 18 h after treatment and TGFβ expression examined by qPCR; results are presented relative to DMSO control. Data represent two to three experiments. (B and D) Data from two to three independent experiments combined. Error bars represent mean ± SEM. *P < 0.05 (unpaired two-tailed Student t test).