Skip to main content
. 2014 Jan 13;111(4):E465–E473. doi: 10.1073/pnas.1320319111

Fig. 4.

Fig. 4.

Thymocyte apoptosis increases intrathymic TGFβ. (A–D) Bim−/− and Bim+/− mice received 0 or 450 rad of γ-irradiation, and thymi were removed 18 h later. (A) FACS examination of AnnexinV and 7AAD staining of thymocytes. (B) Intrathymic amounts of total TGFβ1 in whole thymi determined by ELISA. (C) tgfb1 expression in sorted thymic macrophages determined by qPCR; results are presented relative to unirradiated control of the same genotype. (D) mRNA expression of tgfb1, tgfb2, tgfb3, and Smad7 in whole thymi; results are presented relative to unirradiated control of same genotype. Data represent three experiments, apart from in C, which represents two separate experiments. Error bars represent mean ± SEM. *P < 0.05, **P < 0.005, ***P < 0.0001 (unpaired two-tailed Student t test).