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. 2014 Feb 10;20(5):733–746. doi: 10.1089/ars.2013.5229

FIG. 3.

FIG. 3.

In vitro tumor-killing efficacy of HF-LPLI (200 mW/cm2 at 10 min) in respiration-deficient cells. (A, B) FACS analysis of mitochondrial O2−• generation in ρ0ASTC-a-1 (A) and ρ0A549 (B) cells. The temporal profiles of MitoSOX™ intensities were acquired in cells 1 h after HF-LPLI treatment. (C) ASTC-a-1 and ρ0ASTC-a-1 cells pre-incubated with FCLA received various treatments. Cells were pre-cultured with SOD (50 U/ml) 1 h before HF-LPLI. Data represent the mean±SD (n=5). *P<0.05 versus control; **P<0.01 versus control. (D) FACS analysis of cell apoptosis with annexin V-FITC staining in ASTC-a-1 and ρ0ASTC-a-1 cells 10 h after HF-LPLI treatment. (E) The quantitative analysis of the cell apoptosis ratio shown in (D) and Supplementary Figure S6. The data represent the mean±SD (n=5). **P<0.01 versus control.