FIG. 5.
In vitro tumor-killing efficacy of HF-LPLI (200 mW/cm2 at 10 min) in COX knockdown cells. (A) Effect of HF-LPLI on COX activity in COX subunit knockdown ASTC-a-1 cells generated with two different siRNAs specifically targeting COX II and COX III. The data represent the mean±SD (n=5). (B) FACS analysis of mitochondrial O2−• generation. The temporal profiles of MitoSOX™ intensity were acquired in COX subunit knockdown ASTC-a-1 cells 1 h after HF-LPLI treatment. (C) FACS analysis of cell death with annexin V-FITC/PI double staining in COX subunit knockdown ASTC-a-1 cells 10 h after HF-LPLI treatment. (D) Quantitative analysis of the cell death rate shown in (C) and Supplementary Figure S8. The data represent the mean±SD (n=5). siRNA, small interfering RNA.