FIG. 2.

Detection of HSV-2-specific T cell responses in animals cochallenged with live HSV-2 and SHIV-RT. (A, B) Intracellular cytokine staining (ICS) of HSV-2-specific CD4⁺ or CD8⁺ T cells in peripheral blood mononuclear cells (PBMCs) (A) and lymph node mononuclear cells (LNMCs) (B) of animals cochallenged with UV HSV-2 (n=4) vs. live HSV-2 (n=6) and SHIV-RT. Shown are the means (±SEM) of the percent positive cells [calculated after subtracting background microvesicle (MV) control values from each] detected at the indicated weeks post cochallenge (weeks 15 and 23 for the PBMCs and weeks 4 and 24 for the LNMCs). (C) Polyreactive CD4⁺ and CD8⁺ lymphocytes from PBMCs or LNs relative to single cytokine-reactive cells.