Fig. 2.

Sequence analysis overview. a) Forward and reverse sequence reads obtained from Roche/454 sequencing are joined based on unique SNPs in the overlap region. b) The 530 bp Roche/454 joined sequences are used as a scaffold to assemble the full-length fragmented sequences obtained from Illumina MiSeq analysis. c) Paired-end reads lying outside of the 530 bp scaffold are used to expand the 530 bp sequence. These newly elongated sequences are used as the backbone to expand out the MHC class I transcript over multiple iterations. d) The full-length molecule is generated when iterations no longer expand the sequence length