TABLE 1.

Primers and profiles used for PCR amplification of the pvdhfr and pvdhps genes

Gene	Primer name	Primer sequence (5′–3′)	Mg+2 concn (mM)	PCR program
				Denaturation		Annealing		Elongation		No. of cycles
				Temp (°C)	Time (min)	Temp (°C)	Time (min)	Temp (°C)	Time (min)	1st PCR	2nd PCR
pvdhfr	VDT-OF	ATGGAGGACCTTTCAGATGTATTTGACATT	2	94	1	60	2	72	2	25
	VDT-OR	GGCGGCCATCTCCATGGTTATTTTATCGTG
	VDT-OF	ATGGAGGACCTTTCAGATGTATTTGACATT		94	1	62	2	72	2		30
	VDF-NR	TCACACGGGTAGGCGCCGTTGATCCTCGTG
pvdhps	PVDHPS-OF	ATTCCAGAGTATAAGCACAGCACATTTGAG	3	94	2	60	2	72	1	25
	PVDHPS-OR	CTAAGGTTG ATGTATCCTTGTGAGCACATC
	PVDHPS-NF	AATGGCAAGTGATGGGGCGAGCGTGATTGA		94	2	52	2	72	1		30
	PVDHPS-NR	CAGTCTGCACTCCCCGATGGCCGCGCCACC