FIG 5.
Inhibition of KSHV ori-Lyt-dependent DNA replication with (+)-rutamarin. BCBL-1 cells were transfected with a KSHV ori-Lyt-containing plasmid (pOri-A) and an RTA expression vector (pCR3.1-ORF50). The transfected cells were treated with increasing concentrations of (+)-rutamarin and incubated for 72 h. Hirt DNAs were extracted and digested with KpnI/SacI or KpnI/SacI/DpnI. DpnI-resistant viral replicated DNA (Rep'd DNA) was detected by Southern blotting with 32P-labeled pBluescript plasmid.