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Compounds 1, 2, and 3 inhibit fluconazole-induced Upc2-dependent activity. Cells were grown to exponential phase in the absence (−) and presence (+) of fluconazole (FLC) and each compound (compound 1, 2, or 3) for 6 h at 30°C. Total RNA was extracted, and the mRNA expression levels of ERG11, ERG25, UPC2, and PGK1 from S. cerevisiae (Sc) were determined by qRT-PCR.
