FIG 3.

ImageStream screening of a candidate library. 3D7 trophozoites were treated for 4 h and stained with Fluo-4-AM. The mean area of Fluo-4 fluorescence was then assayed with the ImageStream platform. For panel A, candidate compounds were administered at 10 μM and the resulting mean areas were compared with that of the vehicle (Veh) control. For panel B, hits from the 10 μM screening were selected for rescreening at 1 μM. Three hits were identified at this stage: emetine dihydrochloride (EME), DCB, and QC hydrochloride. Compound name abbreviations are provided in Table 1. Gray bars indicate mean areas significantly different from that of the vehicle control (P < 0.05; n = ≥3). CQ at 3 μM was included in both screenings as a positive control. Data represent means ± the standard errors of the means.