FIG 7.

The DV-destabilizing effects of QC and DCB are recapitulated in field isolates. Two artemisinin-sensitive and two delayed-clearance isolates were treated with QC and DCB for 4 h and then stained with Fluo-4-AM. Confocal microscopy was performed to assess the percentages of parasites with DV-localized fluorescence (black), cytosolic fluorescence (gray), and low or no fluorescence (white). QC retains a potent DV-permeabilizing effect in both the delayed-clearance isolates and one of the artemisinin-sensitive isolates. At least 30 infected erythrocytes were counted per treatment. ***, P < 0.001; **, P < 0.01; *, P < 0.05. Veh, vehicle.