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Bioavailability of hLF in the serum samples of LFKO−/− mice. At different time intervals, blood was collected from mice (n = 5) injected with hLF (300 μg/g of body weight), with or without A. actinomycetemcomitans injection. The amount of hLF in the serum was assessed by ELISA in duplicate experiments for each mouse, and the data are presented as means ± SEM. Asterisks indicate significant difference (P < 0.05) between infection and the hLF-injected group.
