TABLE 2.
Target | Primer sequence (5′→3′) | Annealing temp (°C)a | Amplicon size (bp) |
---|---|---|---|
β-Actin | |||
Sense | ATGTTTGAGACCTTCAACA | 56 | 495 |
Antisense | CACGTCAGACTTCATGATGG | ||
iNOS | |||
Sense | CAGCTGGGCTGTACAAACCTT | 56 | 94 |
Antisense | CATTGGAAGTGAAGCGTTTCG | ||
MPO | |||
Sense | GGAAGGAGACCTAGAGGTTGG | 58 | 127 |
Antisense | TAGCACAGGAAGGCCAATG |
Annealing temperature for PCR.