TABLE 3.
Evaluation of the effects of hLF on serum proinflammatory mediators during A. actinomycetemcomitans bacteremia
| hLF treatment | Concn (pg/ml) of serum inflammatory mediator after treatmenta |
|||||
|---|---|---|---|---|---|---|
| IFN-γ | TNF-α | IL-1β | IL-6 | IL-10 | IL-12p70 | |
| PBS | 29.5 ± 2.1 | 16.5 ± 2.1 | 15.0 ± 1.4 | 27.5 ± 2.1 | 6.4 ± 4.0 | 21.0 ± 1.4 |
| hLF only | 36.0 ± 1.4 | 22.5 ± 0.7 | 18.0 ± 0.0 | 82.0 ± 2.8 | 6.3 ± 1.9 | 26.0 ± 1.4 |
| Bacteria | 1,517 ± 200 | 493 ± 83.4 | 903 ± 115.3 | 34,312 ± 713 | 490 ± 34.0 | 861 ± 89.8 |
| hLF oral feeding | 48.1 ± 0.8* | 6.4 ± 1.3* | 46.72 ± 6.5* | 618 ± 86.3* | 136 ± 15.6* | 106 ± 31.5* |
| Prophylactic | 48.5 ± 2.1* | 20.5 ± 0.7* | 18.0 ± 0.0* | 143.0 ± 4.2* | 32.5 ± 2.8* | 33.5 ± 2.1* |
| Therapeutic | 0.0 ± 0.0* | 2.6 ± 1.2* | 5.7 ± 1.2* | 2.0 ± 1.2* | 4.1 ± 2.0* | 17.1 ± 9.8* |
| Concurrent | 4.2 ± 2.2* | 7.3 ± 1.6* | 7.2 ± 0.6* | 778 ± 12.3* | 238 ± 12.3* | 13.2 ± 0.6* |
a
Serum samples were collected at 48 h postinfection from each group, and the significance (*, P < 0.05) of differences in the reduction of the proinflammatory cytokines analyzed during bacteremia was determined. Different modes of hLF administration (300 μg/g body weight) were analyzed for significant differences from the non-hLF-treated groups.