Figure 1.

Structural properties of APETx2. (A) Sequence alignments of APETx2, with K⁺ channel modulators from sea anemones. Black boxes indicate sequence identities and gray boxes sequence homologies with BDS-I and BDS-II (Anemonia sulcata), and APETx1 (Anthopleura elegantissima). The three disulfide bonds of APETx2, determined by the partial reduction/cyanylation method, are indicated. (B) Expected cleavage fragments of APETx2 and their calculated masses (average mass, M+H+) for singly reduced bonds according to the predicted disulfide bond arrangement. The masses in parenthesis were those observed after cleavage and reduction of peak 2^a and peak 3^b.