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. 2004 Mar 25;23(7):1516–1525. doi: 10.1038/sj.emboj.7600177

Figure 4.

Figure 4

Effect of APETx2 on unitary ASIC3 currents recorded from outside-out patches. ASIC3 currents were recorded from COS transfected cells. Holding potential: −50 mV, pH drop from 7.4 to 6.6. (A) Inhibitory effect of APETx2 (3 μM) on an ASIC3 current recorded from a single excised patch. The current was triggered every minute, and APETx2 was externally applied 30 s before the second pH drop. The inhibition of ASIC3 current was 75±6% (n=4), and reversible. (B) Time-scale magnifications of parts of current trace shown in (A) (see a and b), and amplitude histograms of unitary current recorded before (a) and after (b) the application of APETx2. (C) Statistical analysis of ASIC3 unitary current amplitude measured at −50 mV in the presence and in the absence of APETx2 (4 excised patches, n.s., nonsignificant).