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Effect of sub-growth-inhibitory concentrations of cadazolid (a) and vancomycin (b) on spore formation in C. difficile ATCC 9689. Antibiotics were added to late exponential cultures (t = 6 h) at concentrations representing 0.5× and 1× the agar dilution MIC. Controls received no antibiotics. Total viable cells and spores (after ethanol treatment) were enumerated by colony count. Abbreviations: CDZ, cadazolid; VAN, vancomycin.
