TABLE 2.
Conjugation frequencies of Tn5253
| Donor strain (species) | Pneumococcal recipient | Transfer frequencya | Representative transconjugant |
|---|---|---|---|
| FR67 (S. agalactiae) | FP11 (type 2 rough) | 1.1 × 10−6 | FR81 |
| FR43 (S. gordonii) | FP11 (type 2 rough) | 8.3 × 10−7 | FR61 |
| FR40 (S. pyogenes) | FP11 (type 2 rough) | 6.7 × 10−3 | FR59 |
| FR49 (E. faecalis) | FP11 (type 2 rough) | <1.8 × 10−8 | |
| FR50 (E. faecalis) | FP11 (type 2 rough) | <2.7 × 10−8 | |
| FR22 (S. pneumoniae) | FP11 (type 2 rough) | 1.6 × 10−4 | FR58 |
| FR58 (S. pneumoniae) | FP58 (type 2) | 2 × 10−5 | FR38 |
| FR58 (S. pneumoniae) | HB565 (type 3) | 4.4 × 10−7 | FR39 |
| FR22 (S. pneumoniae) | FP47 (type 4) | 3.2 × 10−5 | FR54 |
| FR58 (S. pneumoniae) | FR55 (type 6) | 1.3 × 10−5 | FR56 |
a
The transfer frequency is expressed as the number of transconjugant CFU per donor CFU, and each result is the mean of at least three mating experiments.