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. 2014 Feb;58(2):966–977. doi: 10.1128/AAC.02183-13

FIG 5.

FIG 5

Protective effect of Pi-PEG 15-20 against emergent pathogens and the MPC. (a to g) Kaplan-Meier survival curves, demonstrating the protective effect of Pi-PEG 15-20 on C. elegans when feeding on several individual pathogens isolated from the stools of critically ill patients (a to f) or MPC (g). n = 8/dish, 3 dishes/group. Results were reproduced in triplicate independent experiments. (h and i) SEM images of MPC grown in 0.1× TY (h) and 0.1× TY plus 5%Pi-PEG 15-20 (i). Red squares indicate the area of magnification shown in the right panels. Red arrows indicate an external synthetic- appearing covering of bacterial cells incubated with Pi-PEG 15-20. (j) IL-8 release by HT-29 cells induced by MPC with or without treatment with 5% PEG 15-20 or 5% Pi-PEG 15-20. n = 3/group. *, P < 0.001; **, P < 0.01 (Student t test).