FIG 6.

Pi-PEG prevents mortality due to the MPC and preserves the intestinal microbiome. (a) Kaplan- Meier survival curves for MCP plus water enema versus MCP plus Pi-PEG enema. n = 5/group. P < 0.001. (b) PCA of microbial taxa based upon protein identification of filtered lysates from cecal contents. Red dots (n = 3) are the nontreated controls, black dots represent mice treated with MPC and a water enema, and green dots represent mice treated with MPC and an Pi-PEG 15–20 enema. The single black dot clustered with the red and green dots in the PCA represents the one mouse in the MPC/water enema group that did not develop sepsis and appeared perfectly healthy on day 7. (c) Taxa analysis, showing the relative distribution of commensal versus pathogenic species by treatment group. (d) IL-6 and IL-12p40 cytokine release by dendritic cells exposed to filtered lysates from mouse cecal contents. n = 5/group. *, P < 0.05l **, P < 0.01.