TABLE 2.

Membrane-embedded acidic amino acids in TM1 of DedA family proteins and selected drug transport proteins^a

Protein name	TM1 amino acid sequenceb	Accession no.	Family
YqjA	FVLFVILFLENGLLPAAFLPGDS	NP417566	DedA
YghB	IVSVVYFVMFATLFLENGLLPAS(FLPGD)c	NP417482	DedA
BB0250	VFFSLLILAGLNVPISEDAIVLMd	NP212384	DedA
MdfA	RLGRQALLFPLCLVLYEFSTYIG(ND)e	P0AEZ0	12-TMS family of the MFS
TetL	LIWLCILSFFSVLNEMVLNVSLP	AAB09024	14-TMS family of the MFS
EmrE	NPYIYLGGAILAEVIGTTLMKFS	P23895	4-TMS family of the SMR family

^aDatabase sequences were analyzed for predicted membrane domains using SOSUI (45). This table is for illustration purposes only and is not a complete list of transport proteins. Abbreviations: TM1, transmembrane region 1; MFS, major facilitator superfamily; SMR, small multidrug resistance.

^bConserved acidic amino acids are shown in bold.

^cYghB D51 is predicted to lie just outside TM1 but is also required for function (see Fig. S3 in the supplemental material).

^dBB0250 is an essential DedA family protein of Borrelia burgdorferi (36). E39 and D40 are each required for BB0250 to complement growth and cell division defects of BC202 (see Fig. S3 in the supplemental material).

^eE26 and D34 both play roles in transport by MdfA (35).