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Western blot analysis of PA expression in vitro. (A) Expression of PA in plasmid-transfected 293 cells. (B) Expression of PA in Ad5-PAopt-infected 293 cells. rPA, recombinant protective antigen; pDC316, shuttle plasmid of the Ad system; pDC316-PAopt, pDC316 containing optimized PA gene; pDC316-PAwt, pDC316 containing wild-type PA gene; Ad5-PAopt, Ad5 expressing humanized PA; Ad5-EGFP, Ad5 expressing enhanced green fluorescence protein; Mock, untransfected or uninfected cells.
