FIG 1.

A 6-kbp region of the MBOVPG45_710 (milA) gene was cloned as two 3-kbp fragments, AB and CD, and subcloned as four 1.5-kbp DNA fragments, A, B, C, and D, with the expressed gene sequences bounded by restriction endonuclease cleavage sites. In addition, two 2-kbp DNA fragments were amplified using primers containing appropriate restriction endonuclease cleavage sites: abF-BamHI (CAACggatccATCAAAGACGTGA [binding site, 144–166]) and abR-SalI (TTCgtcgacGGATTTCGCCT [1931–1950]) and cdF-BamHI (CACTGggatccATCTCGAACATC [3128–3150]) and cdR-SalI (ACAgtcgacCCAGGTTCG [4955–4872]).