SDS-PAGE and Western blot analysis of MilA (226 kDa). Lane 1, Spectra multicolor high-range protein ladder (Fermentas); lane 2, whole-cell proteins of M. bovis strain 3683 stained with Coomassie blue; lane 3, whole-cell proteins of M. agalactiae strain PG2 stained with Coomassie blue; lane 4, Western blot of whole-cell proteins of M. bovis strain 3683 probed with pooled M. bovis-specific calf sera; lane 5, Western blot of whole-cell proteins of M. agalactiae strain PG2 probed with pooled M. bovis-specific calf sera.