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ROC curve for determination of Blastomyces antibody cutoff. The ROC recommended cutoff OD was 0.042 with a sensitivity of 95.1% and a specificity of 93.6%. However, an OD of 0.085 (corresponding to 1.5 EIA units) was chosen for further analysis to increase specificity. At this cutoff level, sensitivity was 87.8% and specificity was 99.2%, the area under the curve was 0.980 (95% confidence interval [CI], 0.946 to 0.995), and the standard error was 0.0122 (P < 0.0001).
