FIG 1.

gB-G eVLPs induce high titers of neutralizing antibodies against HCMV infection of fibroblast and epithelial cells. (A) Fifty percent neutralization endpoint titers are depicted for pooled serum samples from mice (n = 8/group) immunized twice with the indicated antigens. The neutralizing activity of Cytogam served as a positive comparative control. Comparable results were obtained in repeat testing. (B) A second batch of gB-G eVLPs was used to immunize mice, and individual serum samples were compared against those from mice immunized with recombinant gB protein and gB eVLPs for neutralizing activity against epithelial cell infection. The eVLPs induced a significantly higher neutralizing antibody response as determined by Mann-Whitney testing. (C) The frequency of gB-specific T-helper cells was determined for each animal 12 days after a second immunization with recombinant gB or gB-G eVLPs. (B and C) The mean and 95% confidence interval are shown for each group.