FIG 3.

Characterization of gB-G-induced murine monoclonal antibodies. (A) Five gB-G eVLP-induced MAbs (in black) were compared to 2 commercially available neutralizing gB-specific MAbs (catalog no. 54023, Abcam, and catalog no. C9100-21N, United States Biological, for MAbs no. 1 and no. 2, respectively) for reactivity against gB protein by ELISA. (B) Three gB-G eVLP-induced MAbs were tested for reactivity with nonreduced and reduced forms of recombinant gB and gB-G eVLPs by Western blotting. Note that the gB-G form of gB has a lower molecular weight than full-length recombinant gB, expected given the shorter transmembrane and cytoplasmic domains.