FIG 6.

Relative expression across sexual development of mating type loci and pheromone precursors and receptors in the mat A female background. Expression profile are color-coded by their nominal mating type specificity: mat A is in red (dark) and mat a is in green (light). Expression of mating type gene mat a-1 increased 48 h and later after crossing between mat A protoperithecia and mat a conidia, where expression of mat A-1 was generally unchanged across perithecial development. Among pheromone genes, expression of the mat A-specific pheromone precursor gene ccg-4 increased during late perithecial development, and expression of the receptor PRE-2, specific for the peptide pheromone encoded by ccg-4, increased from 48 h. Expression of the receptor PRE-1, specific for the peptide pheromone encoded by mfa-1, was present and steady during perithecial development (Table 2).