TABLE 1.

Comparative performance of MTP and N₆ priming on samples from five development stages in N. crassa sexual reproduction

Primer and parameter	0 h	2 h	48 h	72 h	120 h	Total	Mean	SD
MTP
Total reads obtained	25,344,501	28,901,469	24,546,749	26,620,329	28,459,085	133,872,133	26,774,426.6	1,896,929.4
Mapping to the genome	19,928,814	22,686,873	19,744,776	22,007,918	22,641,520	107,009,901	21,401,980.2	1,455,258.9
Mapping multiple places	2,371,545	3,274,722	1,750,275	2,471,673	1,600,175	11,468,390	2,293,678.0	666,413.2
Mapping ambiguous places	66,261	137,707	58,289	68,038	68,013	398,308	79,661.6	32,697.2
Mapping genes	17,085,018	19,614,422	17,510,480	19,610,346	20,252,575	94,072,841	18,814,568.2	1,417,117.0
N6
Total reads obtained	24,356,818	31,576,781	27,550,897	30,029,099	32,760,914	146,274,509	29,254,901.8	3,360,709.9
Mapping to the genome	21,255,501	28,240,311	22,079,771	25,602,909	29,028,130	126,206,622	25,241,324.4	3,512,362.8
Mapping multiple places	1,043,153	938,159	1,280,859	1,751,283	1,095,907	6,109,361	1,221,872.2	320,958.0
Mapping ambiguous places	90,894	175,281	85,084	89,449	101,099	541,807	108,361.4	37,866.9
Mapping genes	17,441,060	24,592,393	17,694,474	20,588,254	24,579,478	104,895,659	2,097,9131.8	3,517,025.2