TABLE 3.

Phenotyping of knockouts for genes that exhibited upregulated expression during perithecial development^a

Function	Gene (NCU no.)	FGSC	Asexual development		Sexual development
			Mycelium	Conidia	Protoperithecia	Perithecia	Ascus	Ascospores	Paraphyses
Heterokaryotic imcompatibility	het-14 (07511)	11980a 11981A	Normal	Normal	Normal	Normal	Normal	Normal	Normal
	het-6 (04453)	11890a 11891A	Normal	Normal	Dispersalb	Normal	Normal	Normal	Normal
	het-15 (04694)	16715a 16714A	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Mrk signaling	mkr-5 (07449)	13456a 13457A	Normal	Normal	Normal	Youngc	No	No	Normal
	mkr-6 (02919)	15559a 15560A	Normal	Normal	Normal	Normal	Normal	Normal	Normal
RNA silencing	sad-1 (02178)	11151a 11152A	Normal	Normal	Normal	Normal	Normal	No	Normal
	sad-3 (09211)	19729a 19730A	Normal	Normal	Normal	Normal	Normal	No	Normal
	sms-2 (09434)	11160a 11161A	Normal	Normal	Normal	Young	No	No	Normal
	sms-3 (08270)	15892a 15891A	Normal	Normal	Normal	No	No	No	Normal
	qde-1 (07534)	11156a 11157A	Normal	Normal	Normal	Normal	No	No	Normal
RNAib	stc1d (01496)	17464a 17465A	Normal	Normal	Normal	Normal	No	No	Normal

^aCrossings were made between KO strains for each genes from both mating types, and descriptions were based on observation on both sides of the crossing zone.

^bInstead of along the crossing zone, protoperithecia were formed randomly over the plate on sides of both mating types.

^cApparent changes in size and color from protoperithecia to perithecia were observed after crossing, but no development of centrum parachyma.

^dNCU01496 is homologous to stc1 (siRNA to chromatin-1) in fission yeast.