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Assay for the new URC enzyme, Urc8p. For the Urc8p assay, the absorbance was monitored at 340 nm for 10 min, corresponding to the decrease of NADPH. In the inset in the lower left corner, the Urc8p reaction, where malonic semialdehyde is converted into 3-hydroxypropionate with the use of NADPH as the reductant, is presented. No change in absorbance was observed when NADH was used instead of NADPH in a parallel sample.
