TABLE 4.
Log2 fold regulation obtained from the microarray experiment of L. kluyveri genes related to pyrimidine metabolism in response to different sole nitrogen sourcesa
| L. kluyveri name | S. cerevisiae homolog | Sequence ID | Log2 fold regulation in response to: |
|||
|---|---|---|---|---|---|---|
| Uracil | Dihydrouracil | Uridine | Ammonia | |||
| URC1 | None | Contig145.1.A:821 | 9.703 | 4.158 | 10.131 | −2.016 |
| URC2 | YDR520C | Skluy_39.6.2:2 | 1.715 | 0.726 | 0.939 | −0.418 |
| URC3,5 | DUR1,2 | Skluy_36.1.5:5 | 5.264 | 1.554 | 4.326 | −0.406 |
| URC4 | None | Contig31.2.A:628 | 8.410 | 4.048 | 8.012 | −0.501 |
| URC6 | FUR1 | Skluy_1.3.12:12 | 5.465 | 4.622 | 4.502 | 0.080 |
| PYD2 | None | Skluy_13.2.3:3 | 3.721 | 6.908 | 0.839 | −0.078 |
| PYD3 | None | Contig147.1.A:1027 | 3.590 | 9.015 | 2.798 | −0.106 |
| PYD4 | UGA1 | Skluy_29.2.17:17 | 0.585 | 1.213 | −0.441 | −0.941 |
| URC8 | YMR226C | Skluy_76.1.28:28 | 3.819 | 4.984 | 4.161 | −0.201 |
| DAL4 | DAL4 | Skluy_54.3.14:14 | 4.280 | 0.986 | 3.090 | 0.107 |
| DAL5 | DAL5 | Skluy_39.4.2:2 | 5.951 | 1.801 | 5.786 | −0.121 |
| DAL5 homolog | DAL5 | Skluy_40.3.7:7 | −0.513 | 1.105 | −1.614 | −1.609 |
| FUI1 | FUI1 | Skluy_32.2.16:16 | 5.728 | 2.406 | 6.070 | −1.632 |
| FUI1 homolog | FUI1 | SAKL0H03498gb | NA | NA | NA | NA |
| FUR4 | FUR4 | Skluy_182.1.2:2 | 3.890 | 5.903 | 2.406 | −0.211 |
| SAKL0A07480 | Nonec | Skluy_104.1.4:4 | 4.626 | 3.581 | 3.061 | −1.632 |
| DUR3 | DUR3 | Skluy_31.2.8:8 | 4.483 | 1.749 | 3.580 | −0.351 |
a
Cells grown on single-nitrogen-source media were used, and the expression under the four conditions was compared to that in cells grown on media with proline as the nitrogen source. The log2 fold regulation was calculated by logarithmically normalizing the sample/reference ratio of the values recovered from the scanned arrays. NA, not applicable.
b
The oligonucleotide matching this gene could not be identified; therefore, its systematic name found in the Génolevures database was used.
c
Weakly similar to VBA2 (YBR293W).