TABLE 5.
Deleted gene(s)a | Growthb on: |
|||||
---|---|---|---|---|---|---|
Ammonia | Uracil | Uridine | DHU | Allantoin | Urea | |
Single deletion | ||||||
LkFUI1 | +++ | +++ | + | +++ | +++ | +++ |
LkFUI1 hom | +++ | +++ | +++ | +++ | +++ | +++ |
LkDAL5 | +++ | +++ | +++ | +++ | +++ | +++ |
LkDAL5 hom | +++ | +++ | +++ | +++ | +++ | +++ |
LkDAL4 | +++ | +++ | +++ | +++ | +++ | +++ |
LkFUR4 | +++ | +++ | +++ | +++ | +++ | +++ |
LkDUR3 | +++ | +++ | +++ | +++ | +++ | +++ |
SAKL0A07480 | +++ | +++ | +++ | +++ | +++ | +++ |
URC8 | +++ | +/++ | + | +/− | +++ | +++ |
Double deletion | ||||||
ΔLkfur4 ΔLkdal4 | +++ | +++ | +++ | +++ | +++ | +++ |
ΔLkfui1 ΔLkfur4 | +++ | +++ | − | ++ | +++ | +++ |
ΔLkfui1 Δfui1 hom | +++ | +++ | − | +++ | +++ | +++ |
ΔLkfui1 ΔLkdur3 | +++ | +++ | + | ++ | +++ | +++ |
ΔLkfui1 ΔLkdal4 | +++ | +++ | + | +++ | +++ | +++ |
ΔLkfui1 ΔLkdal5 | +++ | +++ | + | +++ | +++ | +++ |
ΔLkfui1 dal5 hom | +++ | +++ | + | +++ | +++ | +++ |
ΔLkfui1 ΔSAKL0A07480 | +++ | +++ | ++ | +++ | +++ | +++ |
ΔLkdur3 ΔSAKL0A07480 | +++ | ++ | ++ | +++ | +++ | +++ |
ΔLkdur3 ΔLkdal5 | +++ | ++ | ++ | +++ | +++ | +++ |
ΔLkdur3 ΔLkdal4 | +++ | ++ | ++ | +++ | +++ | +++ |
Δfui1 hom ΔLkdur3 | +++ | +++ | +++ | ++ | +++ | +++ |
Δfui1 hom ΔLkdal4 | +++ | +++ | +++ | +++ | +++ | +++ |
Δfui1 hom ΔLkdal5 | +++ | +++ | +++ | +++ | +++ | +++ |
Δfui1 hom ΔLkfur4 | +++ | +++ | +++ | +++ | +++ | +++ |
Δfui1 hom ΔSAKL0A07480 | +++ | +++ | +++ | +++ | +++ | +++ |
Δdal5 hom ΔLkdal5 | +++ | +++ | +++ | +++ | +++ | +++ |
ΔLkdal4 ΔLkdal5 | +++ | +++ | +++ | +++ | +++ | +++ |
ΔLkfur4 ΔLkdal5 | +++ | +++ | +++ | +++ | +++ | +++ |
ΔSAKL0A07480 ΔLkdal5 | +++ | +++ | +++ | +++ | +++ | +++ |
Δdal5 hom ΔLkdal4 | +++ | +++ | +++ | +++ | +++ | +++ |
Δdal5 hom ΔLkfur4 | +++ | +++ | +++ | +++ | +++ | +++ |
Δdal5 hom ΔLkdur3 | +++ | +++ | +++ | +++ | +++ | +++ |
Δdal5 hom ΔSAKL0A07480 | +++ | +++ | +++ | +++ | +++ | +++ |
ΔLkfur4 ΔSAKL0A07480 | +++ | +++ | +++ | +++ | +++ | +++ |
ΔLkdal4 ΔSAKL0A07480 | +++ | +++ | +++ | +++ | +++ | +++ |
hom, homolog.
Shading highlights the results which differed from those for the wild-type strain. +++, wild-type-like growth; ++, growth, but less than that of the wild type; +, very limited and slow growth; −, no growth/equal to negative control; NA, not assayed. DHU, dihydrouracil. Growth was inspected after 3 and 6 days.