FIG 2.

C. albicans-induced macrophage lysis is dependent on caspase-1, ASC, and NLRP3. (A and B) Bone-marrow-derived macrophages (BMDM) from C57BL/6 mice (WT) or the indicated knockout strain were prestimulated with LPS and exposed to C. albicans at an MOI of 2:1 for 5 h. (C) BMDM from C57BL/6 or A/J mice were treated with 5 mM glycine or PBS as a control, prestimulated with LPS, and exposed to C. albicans at an MOI of 2:1 for 5 h. LDH and IL-1β were measured from the supernatant of cocultures after 5 h. LDH results were expressed as a percentage of the maximum LDH release as described in the legend to Fig. 1. Each experiment was performed at least twice with similar results, and a representative single experiment is shown. Bar heights are the means of least three technical replicates, and error bars indicate standard deviations. Data were analyzed by Student's t test: **, P ≤ 0.001 compared to control; *, P ≤ 0.05 compared to WT in panels A and B and compared to control in panel C. UND, undetectable.