FIG 2.

Localization of gCYTb5 proteins in Giardia. (A) The cytosolic localization of gCYTb5-I to -IV visualized using immunofluorescence microscopy. The gCYTb5 proteins were visualized using rat anti-HA tag and anti-rat Alexa Fluor 594 (red) antibodies. PDI-2 was detected using mouse anti-PDI-2 and anti-mouse Alexa Fluor 488 (green) antibodies. The nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (blue). DIC, differential interference contrast. (B) Localization of gCYTb5 in subcellular fractions of Giardia using immunoblot analysis. LYS, cell lysate; CYT, cytoplasm; ORG, organellar fraction; Enolase, a cytosolic marker protein; PDI-2, an endoplasmic reticulum marker protein.