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Reduction of gCYTb5-IV by GiOR-1. UV-visible spectra of reduced gCYTb5-IV (dashed lines) were recorded after incubation of oxidized gCYTb5-IV (solid lines) in the presence of NADPH and GiOR-1 for 10 min at 25°C in an anaerobic cuvette. The region between 500 and 600 nm is enlarged in the inset. The wavelengths 413, 426, 529, 559, and 563 nm are indicated by dotted lines.
