FIG 2.

Ssu2 is involved nitrate efflux in oocytes from Xenopus laevis. Oocytes were injected with different amounts of SSU2 cRNA and incubated for 7 days at 17°C. (A) Ssu2-YFP is localized in injected oocytes at the cell surface. (B) The amount of Ssu2-YFP is directly proportional to the amount of cRNA injected. (C) Oocytes injected or not with SSU2 cRNA were preloaded with 0.9 nmol of nitrate and incubated for 15 min. Afterward, nitrate was determined in the extracellular medium. Nitrate (nmol) excreted in 15 min ± SE from 5 independent experiments is shown.